Table 1 Pyroptosis during sepsis-associated acute kidney injury
From: Pyroptosis in sepsis-associated acute kidney injury: mechanisms and therapeutic perspectives
Species | Inducer | Tissue/cell | Time/stage | Pyroptosis assessments | References |
|---|---|---|---|---|---|
Mouse | LPS | Tubular cell | elevated levels of pyroptosis markers NOD-like receptor protein 3 (NLRP3), Caspase1, and gasdermin D (GSDMD) | [37] | |
Mouse | LPS | Mouse kidney tissues、Human umbilical vein endothelial cell | 24H | expressions of renal histopathological、TNF-α, IL-1β, and IL-18, pyroptosis, Caspase-1 and GSDMD-N | [38] |
Mouse | LPS | renal tubular epithelial cells (RTECs) 、HK-2 cell | 24 h | renal histopathological、The levels of pro-inflammatory cytokines IL-1β, TNF-α, and MCP-1 | [39] |
Mouse | CLP | kidney samples、HK-2 cell | 24 h | expressions of NLRP3 、 GSDMD 、Caspase-1、IL-1β IL-18 | [40] |
Mouse | LPS | tubular epithelial cell、caspase-11–/– RTEC cell line | 0, 6, and 12 h | pathologic abnormalities in renal structure、the protein expression of caspase-11, interleukin-1β and Gsdmdc1 | [41] |
Mouse | CLP | Renal tubular epithelial cells (HK-2) | 48 h | the protein expression of NRLP3、c-Caspase1、ASC、IL-1β and IL-18 | [42] |
Mouse | LPS | Mouse kidney tissues、HK-2 cell | 24 h | pathologic abnormalities in renal structure 、The levels of GSDMD-N、TNF-α、IL-6 、IL-1β、NLRP3、caspase-1、ASC、 and IL-18 | [43] |
Mouse | CLP | Mouse kidney tissues | 24 h | pathological changes in renal structure and renal function and the expression of pyroptosis-related indicators Caspase-1, NLRP3, GSDMD, IL-18, and IL-1β | [44] |
Mouse | CLP | Mouse kidney tissues、HK-2 cell | 24 h | The levels of IL-1β, IL-18, NLRP3 inflammasome, cleaved caspase-1, gasdermin D-N levels, and pyroptotic cell number | [45] |
Mouse | CLP | Kidney Tissues 、Human kidney proximal tubular epithelial cells (HK-2) | 24 h | Caspase-1 and GSDMD-N staining in kidney tissues | [46] |
Mouse | LPS | Kidney Tissues 、Human kidney proximal tubular epithelial cells (HK-2) | 24 h | NLRP3, cleaved-Caspase1 and GSDMD-N protein levels | [47] |
Mouse | LPS-induced HK-2 cell model and CLP-induced mouse | Kidney Tissues 、Human kidney proximal tubular epithelial cells (HK-2) | 24 h | The expression of NLRP3, NEK7, ASC, active caspase-1, and N-GSDMD and the inflammatory factors including IL-18, IL-1β, and THF-α | [48] |
mice | LPS | Kidney samples, The human proximal tubule cell line | 24 h | The expression of IL-18, IL-1β, and GSDMD | [49] |